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Thursday, 23rd October 2008 |
| 9.00 - 9.30 h |
Welcome, Introduction to the program
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| 9.30 – 10.45 h |
Theoretical Part
Real-time PCR - theoretical background of technology
- Real-time PCR
- Equipment
- Application
- Sample preparation
|
| 10.45 – 11.00 h |
Coffee Break
|
| 11.00 - 12.30 h |
Microarrays - Introduction into technology
- Real-time PCR – Microarrays
- Equipment
- Application
- Sample preparation
|
| 12.30 h |
Lunch Break
|
| 13.30 – 15.00 h |
Practical Part
DNA Extraction from food-samples
|
| 15.00 – 17.00 h |
Discussion
Guided tour - CONGEN laboratory
|
| 20.00 h |
Dinner at a restaurant
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Friday, 24th October 2008 |
| 9.00 – 11.00 h |
Practical Part
Detection of pathogens by Real time PCR
|
| 11.15 – 12.30 h |
Demonstration - Microarray
|
| 12.30 h |
Lunch Break
|
| 13.30 - 14.00 h |
Theoretical Part
Evaluation of results
|
| 14.00 – 15.00 h |
Stephan Hühn, guest speaker "Development and validation of a DNA microarray for characterization and typing of Salmonella isolates"
|
| 15.15 – 16.30 h |
Regulatory framework of molecular diagnostics
International standards
Open discussion |