Detection Methods

Agence Francaise de Securite Sanitaire des Aliments, France

  • Advising on how to perform microbiological sampling of surfaces
  • Interpretation of results from microbial sampling of surfaces
  • Advising in case of repeated detection of a same clone of Listeria monocytogenes in ready-to-eat food (persistence of L. monocytogenes in the environment)

Agricultural University of Athens, Greece 

  • Bacteriocins, antimicrobial screening
  • Food matrix applications
  • Predictive Microbiology
  • Modelling
  • Quantitative Microbiological Risk Assessment
  • Qualitative Microbiological Risk Assessment

CONGEN Biotechnologie GmbH, Germany

  • Realtime PCR for GMO, ALLERGEN, PATHOGEN detection and animal and plant species identification
  • Microarray analysis for monitoring virulence potential and virulence gene expression 
  • Microarray analysis for testing probiotic potential and probiotic activity for selected strains

Corvinus University of Budapest, Hungary

  • Application of PCR-based molecular techniques for detection of food-borne pathogens
  • Validation of molecular techniques

Danisco A/S, Denmark

  • Screening of bacterial isolates for antimicrobial activity
  • iHTA (intelligent high-throughput assessment)
  • Rapid epifluorescence method
  • Testing of bacterial strains
  • In-vitro and In-situ
  • Pilot Plant for testing in relevant food models
  • Campylobacter jejuni
  • Salmonella thypimurium

Forschungszentrum Karlsruhe, Germany

  • Interpretation of results from microbial sampling of drinking water used during food production.
  • Microbiological control of drinking water used during food production.
  • Advanced technologies: DGGE, PCR, Real Time PCR, Sequencing.
  • Application of end point PCR and realtime TaqMan PCR techniques for detection and quantification of the following food-borne pathogens in water used during food processing: Mycobacterium avium subsp. paratuberculosis, Salmonella, Enterococci, Campylobacter jejuni, Pseudomonas aeruginosa and Listeria monocytogenes

Ghent University, Belgium

  • Food microbiology analytics under accreditation system: Spoilage organisms
  • total aerobic and anaerobic count, spores of aerobic and anaerobic organisms
  • yeasts and molds, osmophilic yeasts
  • lactic acid bacteria
  • sulphite reducing clostridia and spores
  • Foodborne pathogens
  • Salmonella spp.
  • Listeria spp. and Listeria monocytogenes
  • Campylobacter jejuni/coli
  • Escherichia coli O157
  • coagulase positive Staphylococcus aureus
  • Bacillus cereus and spores
  • Clostridium perfringens and spores
  • Enterobacter sakazakii
  • Detection of Staphylococcus aureus and Bacillus cereus toxins
  • Microbial challenge testing
  • Research support (Follow-up of recent developments in officially recognized ISO methods; Optimization and evaluation of alternative (rapid) detection methods for foodborne pathogens; Characterization of emerging pathogens: prevalence, growth and survival, virulence)
  • Microbial analysis of foods in accredited (ISO 17025) laboratory
  • Microbial ecology of food: definition of microbial parameters of interest being both foodborne pathogens and food spoilage indicators with regard to a particular food

Institute Technique Francais des Fromages, France

  • Evaluation of growth comportement of pathogenic bacteria in dairy foods (challenge tests, predictive microbiology)
  • Quantitative Analysis of Dairy Data (QADD) in order to improve the quality of collected milk

Max Rubner Institute (previous federal research Centre for Nutrition and Food) Karlsruhe

  • Detection and quantification of foodborne pathogens including biochemical and molecular methods (e.g., PCR, qRT-PCR)
  • Investigations into food ecology, biodiversity studies using fingerprinting methods and numerical (cluster) analysis (rep-PCR, ARDRA, RAPD, PFGE)
  • Molecular identification techniques including 16S sequencing and sequence based phylogenetic analyses
  • Molecular detection methods for mycotoxin producing fungi
  • Expression systems (Real Time PCR, microarray) for mycotoxin producing fungi
  • Molecular typing and characterization of ochratoxin A producing Penicillia

Profos AG, Germany

  • Listeria Capture Kit for PCR, 22 hours time-to-result (192 Eur for 48 tests)
  • Listeria Capture Kit for Plating, 48 hours time-to-result (180 Eur for 100 tests)

Technical University of Denmark

  • Discussion on methods used for quantifying bacteria on surfaces; in the laboratory and in industry

University of Udine/Torino, Italy

  • Microbiological control of foods and environment
  • Direct molecular analysis for the detection of pathogens

 

vermicon AG, Germany 

  • Application of molecular-biological technologies for the fast and specific analysis of microorganisms – including uncultivable microorganisms
  • Advanced technologies: FISH, DGGE, PCR, Sequencing, Cloning
  • Offer of products and services in the field of food, drinks, water, environment; detection of pathogens, probiotics, any organism of interest
  • Offering comprehensive molecular-biological and microbiological services and research tools for industrial as well as scientific customers worldwide
  • Development and Distribution of specific testkits for in-house analysis of bacteria and yeasts in food, drinks, water and wastewater; based on the vermicon identification technology (VIT®).
    Available testkits for food:
    • VIT-Salmonella
    • VIT-Listeria
    • VIT-Staphylococcus
    • VIT-E. coli/Coliforms
    • VIT-Enterobacter sakazakii
    • VIT-Campylobacter

    Veterinary Research Institute, Czech Republic

    • Culture of ethiological agent of paratuberculosis (Mycobacterium avium subsp. paratuberculosis)
    • Detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk and milk products by real time qPCR
    • Detection and quantification of Mycobacterium avium subsp. paratuberculosis in tissues and meat by real time qPCR
    • Expression profiling of Mycobacterium avium subsp. paratuberculosis in milk derived matrices

  • More than 10 years of experience in the field of microbiological analysis

Dominik Koenig, - last update:19 November 2008
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