Detection Methods
Agence Francaise de Securite Sanitaire des Aliments, France
- Advising on how to perform microbiological sampling of surfaces
- Interpretation of results from microbial sampling of surfaces
- Advising in case of repeated detection of a same clone of Listeria monocytogenes in ready-to-eat food (persistence of L. monocytogenes in the environment)
- Bacteriocins, antimicrobial screening
- Food matrix applications
- Predictive Microbiology
- Modelling
- Quantitative Microbiological Risk Assessment
- Qualitative Microbiological Risk Assessment
- Realtime PCR for GMO, ALLERGEN, PATHOGEN detection and animal and plant species identification
- Microarray analysis for monitoring virulence potential and virulence gene expression
- Microarray analysis for testing probiotic potential and probiotic activity for selected strains
- Application of PCR-based molecular techniques for detection of food-borne pathogens
- Validation of molecular techniques
- Screening of bacterial isolates for antimicrobial activity
- iHTA (intelligent high-throughput assessment)
- Rapid epifluorescence method
- Testing of bacterial strains
- In-vitro and In-situ
- Pilot Plant for testing in relevant food models
- Campylobacter jejuni
- Salmonella thypimurium
- Interpretation of results from microbial sampling of drinking water used during food production.
- Microbiological control of drinking water used during food production.
- Advanced technologies: DGGE, PCR, Real Time PCR, Sequencing.
- Application of end point PCR and realtime TaqMan PCR techniques for detection and quantification of the following food-borne pathogens in water used during food processing: Mycobacterium avium subsp. paratuberculosis, Salmonella, Enterococci, Campylobacter jejuni, Pseudomonas aeruginosa and Listeria monocytogenes
- Food microbiology analytics under accreditation system: Spoilage organisms
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total aerobic and anaerobic count, spores of aerobic and anaerobic organisms
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yeasts and molds, osmophilic yeasts
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lactic acid bacteria
- sulphite reducing clostridia and spores
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Foodborne pathogens
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Salmonella spp.
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Listeria spp. and Listeria monocytogenes
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Campylobacter jejuni/coli
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Escherichia coli O157
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coagulase positive Staphylococcus aureus
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Bacillus cereus and spores
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Clostridium perfringens and spores
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Enterobacter sakazakii
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Detection of Staphylococcus aureus and Bacillus cereus toxins
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Microbial challenge testing
- Research support (Follow-up of recent developments in officially recognized ISO methods; Optimization and evaluation of alternative (rapid) detection methods for foodborne pathogens; Characterization of emerging pathogens: prevalence, growth and survival, virulence)
- Microbial analysis of foods in accredited (ISO 17025) laboratory
- Microbial ecology of food: definition of microbial parameters of interest being both foodborne pathogens and food spoilage indicators with regard to a particular food
- Evaluation of growth comportement of pathogenic bacteria in dairy foods (challenge tests, predictive microbiology)
- Quantitative Analysis of Dairy Data (QADD) in order to improve the quality of collected milk
- Detection and quantification of foodborne pathogens including biochemical and molecular methods (e.g., PCR, qRT-PCR)
- Investigations into food ecology, biodiversity studies using fingerprinting methods and numerical (cluster) analysis (rep-PCR, ARDRA, RAPD, PFGE)
- Molecular identification techniques including 16S sequencing and sequence based phylogenetic analyses
- Molecular detection methods for mycotoxin producing fungi
- Expression systems (Real Time PCR, microarray) for mycotoxin producing fungi
- Molecular typing and characterization of ochratoxin A producing Penicillia
- Listeria Capture Kit for PCR, 22 hours time-to-result (192 Eur for 48 tests)
- Listeria Capture Kit for Plating, 48 hours time-to-result (180 Eur for 100 tests)
- Discussion on methods used for quantifying bacteria on surfaces; in the laboratory and in industry
- Microbiological control of foods and environment
- Direct molecular analysis for the detection of pathogens
vermicon AG, Germany
- Application of molecular-biological technologies for the fast and specific analysis of microorganisms – including uncultivable microorganisms
- Advanced technologies: FISH, DGGE, PCR, Sequencing, Cloning
- Offer of products and services in the field of food, drinks, water, environment; detection of pathogens, probiotics, any organism of interest
- Offering comprehensive molecular-biological and microbiological services and research tools for industrial as well as scientific customers worldwide
- Development and Distribution of specific testkits for in-house analysis of bacteria and yeasts in food, drinks, water and wastewater; based on the vermicon identification technology (VIT®).
Available testkits for food:
- VIT-Salmonella
- VIT-Listeria
- VIT-Staphylococcus
- VIT-E. coli/Coliforms
- VIT-Enterobacter sakazakii
- VIT-Campylobacter
- Culture of ethiological agent of paratuberculosis (Mycobacterium avium subsp. paratuberculosis)
- Detection and quantification of Mycobacterium avium subsp. paratuberculosis in milk and milk products by real time qPCR
- Detection and quantification of Mycobacterium avium subsp. paratuberculosis in tissues and meat by real time qPCR
- Expression profiling of Mycobacterium avium subsp. paratuberculosis in milk derived matrices
- More than 10 years of experience in the field of microbiological analysis
Dominik Koenig, - last update:19 November 2008