Workshop Berlin 23. - 24. October 2008

Real-time PCR and Microarrays for the detection and risk analysis of food-pathogens

Agenda

Location: Congen Biotechnologie GmbH / Gläsernes Labor
Robert-Rössle-Str. 10
13125 Berlin / Germany
Time: 23rd to 24th October 2008


Thursday, 23rd October 2008

9.00 - 9.30 h

Welcome, Introduction to the program

 

9.30 – 10.45 h

Theoretical Part

Real-time PCR - theoretical background of technology

  • Real-time PCR
  • Equipment
  • Application
  • Sample preparation
10.45 – 11.00 h

Coffee Break

 

11.00 - 12.30 h 

Microarrays - Introduction into technology

  • Real-time PCR – Microarrays
  • Equipment
  • Application
  • Sample preparation
12.30 h

 Lunch Break

 

13.30 – 15.00 h

Practical Part

DNA Extraction from food-samples

 

15.00 – 17.00 h

Discussion
Guided tour - CONGEN laboratory

 

20.00 h

 Dinner at a restaurant

 

 

Friday, 24th October 2008

9.00 – 11.00 h

Practical Part

Detection of pathogens by Real time PCR

 

11.15 – 12.30 h 

Demonstration - Microarray

 

12.30 h

Lunch Break

 

13.30 - 14.00 h

Theoretical Part

Evaluation of results

 

14.00 – 15.00 h

Stephan Hühn, guest speaker "Development and validation of a DNA microarray for characterization and typing of Salmonella isolates"

 

15.15 – 16.30 h Regulatory framework of molecular diagnostics
International standards

Open discussion

 

Dominik Koenig, - last update:9 July 2008


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