Culture Independent Techniques, Microarrays and Resuscitation and Enrichment Methods: Principles and Applications : 17. april 2008 - 17. april 2008

 

 

Food safety is of fundamental importance to the European consumer, the food industry and the economy. The impact on trade and competitiveness is very substantial. Despite significant investment, the incidence of food derived disease still increases in the EU. The EU FP6 project `PathogenCombat` attacks this pan-European problem through a holistic, multidisciplinary approach towards threats from new/emerging pathogens in the entire food chain. The impact in Europe of PathogenCombat with 44 Partners from 17 countries is bound to be very significant. PathogenCombat will contribute strongly to improve the effectiveness and uniformity in reducing the prevalence of foodborne pathogens in European food.

 

Programme

09.30—10.00:       Welcome and Introduction
                              Prof. Luca Cocolin, University of Torino, Italy

10.00—12.00:       Session I

                             Culture Independent methods 
   

                             Prof. Luca Cocolin, University of Torino, Italy
                             Dr. Kalliopi Rantsiou, University of Torino, Italy

                             Dr. Claudia Beimfohr, vermicon AG, Germany

• Denaturating Gradient Gel Electrophoresis (DGGE), principles and aplications
• Fluorescence In Situ Hybridization (FISH), principles and applications
• Quantitative PCR (qPCR), principles and applications
  

12.00—13.00:      Session II

                            Microarrays

 

                            Dr. Matthias Kuhn, ConGen Biotechnology, Germany

• Introduction to general microarray technologies

13.00—14.00:      Lunch Break

 

14.00—15.00:      Session II

                            Microarrays (continued)

• Development of an DNA microarray for characterization of pathogens
• Preanalytics, sample preparation, labeling, hybridization and data analysis
  

15.00—17.00:      Session III

                            Resuscitation and Enrichment Methods

 

                            Vicky Jasson, University of Gent

• Challenges for detection of pathogens in food: sub-lethal injury and low numbers
  Prof. Peter Raspor, University of Ljublijana, Slovenia
• Appropriate resusciation and enrichment as a prerequisite for reliable detection
• Tools available to enhance sample preparation in rapid detection methods
  

17.00:                Concluding Remarks

 

 

 

Information and Registration

 

Please find further information in the >>workshop flyer

 

Registration is mandatory. Please use the >>registration form

 

Attached, you can also find a >>city map